Can the Cogent NGS Immune Profiler Software analyze only Read1 sequencing data?
Can the Cogent NGS Immune Profiler Software analyze only Read1 sequencing data?
No. Read 2 is essential for the Cogent NGS Immune Profiler Software analysis, since it carries UMIs as shown in the SMARTer Human TCR a/b Profiling Kit v2 User Manual - Figure 4. SMARTer human TCR profiling v2 library structure. Cogent NGS Immune Profiler Software requires the presence of UMIs for the analysis.